We would like to submit the enclosed manuscript entitled "GDNF Acutely Modulates Neuronal Excitability and A-type Potassium Channels in Midbrain Dopaminergic Neurons", which we wish to be considered for publication in Nature Neuroscience.
GDNF has long been thought to be a potent neurotrophic factor for the survival of midbrain dopaminergic neurons, which are degenerated in Parkinson’s disease. In this paper, we report an unexpected, acute effect of GDNF on A-type potassium channels, leading to a potentiation of neuronal excitability, in the dopaminergic neurons in culture as well as in adult brain slices. Further, we show that GDNF regulates the K+ channels through a mechanism that involves activation of MAP kinase. Thus, this study has revealed, for the first time, an acute modulation of ion channels by GDNF. Our findings challenge the classic view of GDNF as a long-term survival factor for midbrain dopaminergic neurons, and suggest that the normal function of GDNF is to regulate neuronal excitability, and consequently dopamine release. The
se results may also have implications in the treatment of Parkinson’s disease. Due to a direct competition and conflict of interest, we request that Drs. XXX of Harvard Univ., and YY of Yale Univ. not be considered as reviewers. With thanks for your consideration,
We would like to submit the enclosed manuscript entitled "Ca2+-binding protein frequenin mediates GDNF-induced potentiation of Ca2+ channels and transmitter release", which we wish to be considered for publication in Neuron.
We believe that two aspects of this manuscript will make it interesting to general readers of Neuron. First, we report that GDNF has a long-term regulatory effect on neurotransmitter release at the neuromuscular synapses. This provides the first physiological evidence for a role of this new family of neurotrophic factors in functional synaptic transmission. Second, we show that the GDNF effect is mediated by enhancing the expression of the Ca2+-binding protein frequenin. Further, GDNF and frequenin facilitate synaptic transmission by enhancing Ca2+ channel activity, leading to an enhancement of Ca2+ influx. Thus, this study has identified, for the first time, a molecular target that mediates the long-term, synaptic action of a neurotrophic factor. Our findings may also have general implications in the cell biology of neurotransmitter release.
le Cover Letter[the example used is the IJEB]Case 3
Sample Cover Letter[the example used is the IJEB]
Dear Editor of the [please type in journal title or acronym]:
Enclosed is a paper, entitled "Mobile Agents for Network Management." Please accept it as a candidate for publication in the [journal title]. Below are our responses to your submission requirements.
1. Title and the central theme of the article.
Paper title: "Mobile Agents for Network Management." This study reviews the concepts of mobile agents and distributed network management system. It proposes a mobile agent-based implementation framework and creates a prototype system to demonstrate the superior performance of a mobile agent-based network over the conventional client-server architecture in a large network environment.
2. Which subject/theme of the Journal
the material fits
New enabling technologies (if no matching subject/theme, enter 'Subject highly related to [subject of journal] but not listed by [please type in journal title or acronym])
3. Why the material is important in its field and why the material should be published in [please type in journal title or acronym]?
The necessity of having an effective computer network is rapidly growing alongside the implementation of information technology. Finding an appropriate network management system
has become increasingly important today's distributed environment. However, the conventional centralized architecture, which routinely requests the status information of local units by the central server, is not sufficient to manage the growing requests. Recently, a new framework that uses mobile
agent technology to assist the distributed management has emerged. The mobile agent redu
ces network traffic, distributes management tasks, and improves operational performance. Given today's bandwidth demand over the Internet, it is important for the [journal title/acronym] readers
to understand this technology and its benefits. This study gives a real-life example of how to use mobile agents for distributed network management. It is the first in the literature that reports the analysis of network performance based on an operational prototype of mobile agent-based distributed
network. We strongly believe the contribution of this study warrants its publication in the [journal title/acronym].
4. Names, addresses, and email addresses of four expert referees.
Prof. Dr. William Gates
Chair Professor of Information Technology
321 Johnson Hall
Lancaster, NY 00012-6666, USA
phone: +1-888-888-8888 - fax: +1-888-888-8886 e-mail: firstname.lastname@example.org
Expertise: published a related paper ("TCP/IP and OSI: Four Strategies for Interconnection") in CACM, 38(3), pp. 188-198.
Relationship: I met Dr. Gate only once at a conference in 1999. I didn't know him personally.
Assoc Prof. Dr. John Adams
Director of Network Research Center
College of Business Australian University
123, Harbor Drive Sydney,
phone: +61-8-8888-8888 - fax: +61-8-8888-8886
Expertise: published a related paper ("Creating Mobile Agents") in IEEE TOSE, 18(8), pp. 88-98.
Relationship: None. I have never met Dr. Adams.
Assoc Prof. Dr. Chia-Ho Chen
Chair of MIS Department
College of Management
888, Putong Road
Keelung, Taiwan 100
phone: +886-2-8888-8888 - fax: +886-2-8888-8886
Expertise: published a related paper ("Network Management for E-Commerce") in IJ Electronic Business, 1(4), pp. 18-28.
Relationship: Former professor, dissertation chairman.
Mr. Frank Young
Partner, ABC Consulting
888, Seashore Highway
Won Kok, Kowloon
phone: +852-8888-8888 - fax: +852-8888-8886
Expertise: Mr. Young provides consulting services extensively to his clients regarding network management practices.
Relationship: I have worked with Mr. Young in several consulting projects in the past three years.
Finally, this paper is our original unpublished work and it has not been submitted to any other journal for reviews.